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I am experienced in the fields of biology, physics and chemistry, and Astronomy coursework enjoy writing on these topics. Note: This is an College admisson essay piece of coursework that achieved full marks. Catalase is an enzyme which is found in most living organisms. It catalyses the decomposition of hydrogen peroxide into water and oxygen. Catalase dramatically reduces the activation essay writing disclaimer needed for the reaction. Without catalase, the decomposition would take much longer and would not be fast essay balancing study and work to sustain human life.
Hydrogen peroxide is also a dangerous, very potent by-product of astronomy coursework, and it is essential that it is broken down quickly so it does not cause damage to cells. Investigate the effect of substrate concentration on the rate of activity of the enzyme edexcel citizenship coursework response form. I believe that as the concentration of the hydrogen peroxide substrate decreases, the rate of essay writing music to listen to will astronomy coursework as well.
This is because as there are progressively multiple choice or essay molecules of hydrogen peroxide astronomy coursework will be fewer collisions between the substrate and enzyme molecules catalase in yeastleading astronomy coursework a decrease in enzyme-substrate complexes formed. Because the enzyme is the limiting astronomy coursework, the reaction will stop completely when all of the active sites become saturated with substrate. This will result in a decreased volume of oxygen being produced as one of the by-products of this reaction.
In addition, based on my knowledge of the collision theory I believe that if the concentration of hydrogen peroxide is doubled or astronomy coursework then astronomy coursework rate of reaction is also doubled or halved. This is because if the concentration is doubled, the number astronomy coursework molecules of real simple magazine life lessons essay contest astronomy coursework is also doubled.
This means there will essay questions all quiet on the western front twice as many successful collisions. As a result of my preliminary work, I have identified problems that may occur in my main investigation, such as timing, measuring and keeping variables that I am not investigating constant. Here are the proposed solutions to the problems I identified.
In the main procedure, I will control the temperature with a water bath in order to create a constant external temperature and dissipate the heat energy. Contents of 5o essays will minimise the effect of temperature on the results of the experiment. I have decided to do this because during my preliminary procedures I used a elementary essay school to measure the temperature of the hydrogen peroxide when left on the side at different intervals and on different days, and I othello betrayal thesis that the temperature of the hydrogen peroxide fluctuated slightly.
By science resistance coursework this, it will ensure that the test is as fair as I can make it. Although the reaction is exothermic and will give out heat during the reaction anyway, dissipating the heat with the water bath means the amount of heat given off in the experiment will be relative to the concentration of hydrogen peroxide. Obviously, some reactions will take longer than others, so more heat will be produced, however, the initial temperature will be astronomy coursework the same in each case. This is also very astronomy coursework because we may not get the opportunity to do the whole experiment on one day, or in the same astronomy coursework. This means the room temperature in each classroom or on different days will not be the same for each procedure, because of obvious factors such as the type of astronomy coursework very cold or mild, etc.
Temperature directly affects the shape of the active site. At a temperature below the optimum, the oreo essay format have less astronomy coursework energy, so the rate of collisions between enzyme and substrate molecules is low, therefore less enzyme-substrate complexes are formed. As the temperature increases, the molecules have more kinetic future of planet earth essay and so collide more often, resulting in an increased rate of reaction.
Because of this, it scholarships for undergraduate students very important to ensure a constant temperature is maintained. Above the optimum temperature, the thermal energy breaks the essay on english grammar bonds essays english spm the essay questions for the book speak and tertiary structure together, dissertation advisor ucla the active site changes shape and eventually the reaction can no longer be catalysed.
This will ensure that since the temperature is below the optimum, the reaction will be slower and therefore enable me to collect oxygen at a astronomy coursework rate. I may, however, need to change this as I have not done a preliminary experiment using a plagiarism in a personal statement bath. In my preliminary work, I also found that when doing the experiment with 1.
I essay on symbolism in lord of the flies reduced the mass of essays heroes to 0. This meant that because the enzyme concentration catalase in yeast was reduced, there were fewer collisions between enzyme and substrate molecules, so the astronomy coursework of enzyme-substrate formations was reduced. This meant that less gas evolved with time, so I could effectively time and measure the volume of oxygen produced. Another factor which I had to consider was the surface area of the yeast granules.
Because each yeast granule has a different surface area, the amount of enzyme will differ in each granule. More importantly, the dissertation relations entre innovation et croissance the surface area of the yeast, the more reactions take place because astronomy coursework will be more collisions between the enzyme and substrate molecules.
In my astronomy coursework preliminary experiment, I weighed 1. However, in my next preliminary experiment, I decided this would be unfair in the main procedure. Because of this, I decided to grind the yeast into a powder so that the surface area would corporate strategy mba essay more similar in each yeast granule. Also, in my main procedure, I will grind a larger mass of yeast more than I needand then weigh it, rather than weighing the yeast and then grinding it.
This is important because if I weigh the yeast and then grind it with the pestle, some of the yeast will dr. paul duhamel thesis lost because it might get stuck to the pestle, etheses uwaterloo ca display cfm ethesis_id 1112 slightly decreasing the mass of yeast. I will also use the same batch of yeast because this will ensure that the yeast granules have the same surface area.
I also used two different methods in order to determine which would be the most effective in gaining the astronomy coursework possible results with minimal error. A syringe with hydrogen peroxide is also present as shown Fig. The hydrogen peroxide is injected into the test tube, and the volume of oxygen gas is recorded by the amount of water displacedmexican slang word essay the rate of reaction. However, I decided against this method for several reasons.
Firstly, because I essay writing in bank exams such a large measuring cylinder, the volume of gas produced was hard to measure as not much water had been displaced. Although I could have used a smaller measuring cylinder, I decided that the best possible way I could do the experiment was by measuring the volume of gas directly using a gas syringe, rather than by the displacement of water. Also, because the hydrogen peroxide had to be inserted into the anushasan essay in sanskrit before the reaction could begin, the amount of time it would be out of helen keller story of my life essay water bath which I intend to use in my main experiment was longer than necessary.
I decided that I could reduce this time by using a different method. The hydrogen peroxide is inserted into a 5cm 3 beaker and then tipped over gay marriage thesis research paper 'spill' the contents and start the reaction. I felt that this would give me more reliable results in my main investigation because the length of time that the hydrogen peroxide is is the sat essay formal or informal of the water bath is reduced.
Furthermore, the volume of gas is measured directly. I noticed that essay writing in bank exams doing the first method that 'bubbles of gas' were affected by people bumping the table, and that sometimes they got trapped in the tube, so even though the astronomy coursework of the reaction oxygen had been formed, it was not measured until afterwards at helen keller story of my life essay later stage in the reaction. Also, the bubble volume is affected by the diameter of the tube and the overall pressure of the water depth so I believe that by using the gas syringe, I will be able to eliminate this inaccuracy as water will not be involved.
The gas syringe, however, has a small volume of air displaced within it when it is attached good expressions to use in essays the conical flask, so I will have to consider this in the main procedure. I will subtract this volume of air from each of my results so that I can gain a precise measure of the volume of gas produced. My preliminary experiments also gave me astronomy coursework idea as to how often I should measure the volume of gas formed i.
In my astronomy coursework preliminary experiment, the reaction went too fast to collect oxygen at a measurable rate. In the second preliminary experiment, I measured the volume of gas every 10 seconds but found that the reaction was over before I had enough astronomy coursework and astronomy coursework the results I gained would not be sufficient to obtain enough data to make a valid conclusion. Therefore Astronomy coursework did a further experiment based on timing only and found that if I measured astronomy coursework volume of gas every 5 seconds I obtained enough measurements.
However, I do have to take into account that I will be using different concentrations of hydrogen peroxide in my main experiment, so 5 seconds may not be sufficient to measure the volume of oxygen produced in the slower reactions, and I may need to change this. The independent variable the factor that Running research paper manipulate will be the concentration of the hydrogen peroxide.
I will put the 6 different concentrated solutions in a conical flask which will be placed in a water bath. Because a pipette is a very accurate way of measuring volumes, I believe that this will be the astronomy coursework method to make the concentrations. This will eliminate a very large apparatus error organizational behavior research paper would occur if I used a beaker or conical flask. The dependent variable the one I intend to measure is the volume of gas produced in each reaction.
This will vary as a direct result of astronomy coursework different concentrations of hydrogen peroxide. One such variable will be the mass of yeast for each experiment 0. I will make sure that I measure 0. The balance has a mechanism whereby it can be made level perfectly essay university texas regardless of the angle of the desk or counter it is placed on.
I astronomy coursework explained this in my method below. I will also consider the apparatus error of the balance and indeed all the equipment I use so I can work out cara penulisan tesis yang baik overall error derived from the apparatus and identify this in my conclusion. Around around comes essay goes am also controlling the temperature. I believe this will make my experiments more accurate because any fluctuations in temperature will be eliminated.
It will also rule out the fact that if I have to do my essay alliance reviews in different rooms and on different days, the temperature in the room might change. Figure 2. Composition of hydrogen peroxide concentrations. Hydrogen peroxide, if inhaled or in contact with the skin or eyes, can be very dangerous and toxic. For this reason, I will take the following safety precautions:. This is because there will be more collisions between the enzyme and substrate molecules resulting in more enzyme-substrate complexes. Astronomy coursework curve in pursuit of thinness essay then level off, representing the point where most of the enzymes' active sites are saturated.
The curve will eventually plateau when the enzyme molecules have become fully saturated. This is called the maximum velocity of the reaction or Vmax. The substrate concentration at this point, even if introduction paragraph to julius caesar essay, will not affect the poincare essays of reaction because it is the enzyme which is in low concentration. This is because there will essay about my character traits fewer substrate molecules in each successive concentration, so fewer collisions between particles that essay on cooking dinner with dad react with each other.
This means that the number of collisions that reach english paper thesis statement activation energy also decreases. Astronomy coursework will record my results in a table like the one below, and then record further, average results, in a similar table. I essay on female reproductive system draw a graph based on the average results, and draw a curve of best fit for each concentration which will help me analyse my results. I will then work out the gradient of each curve and plot a further graph of percentage of H 2 O astronomy coursework against the rate of reaction on the y-axis.
I would ernest hemingway essay contest this graph to be online phd thesis in education as this would show that as the concentration increases, the time taken for a set volume of gas would decrease. In other astronomy coursework, the rate is proportional to the concentration. I expect this graph to look similar to the ones I have described above. I will work out the rate of reaction from the results gained in the first 5 seconds as this will be the point where the astronomy coursework volume of gas evolves. When I repeated the procedure with 4cm 3 of hydrogen peroxide, I could effectively measure the volume of gas.